甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白
Active Parathyroid Hormone Related Protein (PTHrP)
PTHLH; HHM; PTHR; PLP; Osteostatin; Parathyroid Hormone Like Hormone
- 編號UAPA819Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點10.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant PTHrP, Mouse.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
PTHrP (Parathyroid Hormone Related Protein ) is a neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. A binding ELISA assay was conducted to detect the association of PTHrP with PTHR1. Briefly, PTHrP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL PTHrP were then transferred to PTHR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PTHrP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of PTHrP and PTHR1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA819Mu01 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA819Mu02 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA819Mu01 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UCPA819Mu21 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA819Mu22 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA819Mu31 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)鑰孔血藍蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA819Mu01 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA819Mu02 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA819Mu08 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA819Mu | 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA819Mu | 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA819Mu | 甲狀旁腺激素相關(guān)蛋白(PTHrP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA819Mu11 | 甲狀旁腺激素相關(guān)蛋白(PTHrP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Clinical and Experimental Metastasis | Characterization of a new renal cell carcinoma bone metastasis mouse model [PubMed: 20443133] |
| Stem Cells and Development | Temporal activation of β-catenin signaling in the chondrogenic process of mesenchymal stem cells affects the phenotype of the cartilage generated. [PubMed: 22133004] |
| International journal of biological sciences | PTHrP Expression in Human MDA-MB-231 Breast Cancer Cells Is Critical for Tumor Growth and Survival and Osteoblast Inhibition [PubMed: PMC3753447] |
| Investigational New Drugs | Tetrahydrofurofuran-type lignans inhibit breast cancer-mediated bone destruction by blocking the vicious cycle between cancer cells, osteoblasts and osteoclasts [Pubmed: 23673814] |
| Toxicology and Applied Pharmacology | Betulinic acid, a bioactive pentacyclic triterpenoid, inhibits skeletal-related events induced by breast cancer bone metastases and treatment [Pubmed:24463094] |
| Arch Gynecol Obstet. | Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study [Pubmed:24943060] |
| PLoS One | 15-Deoxy-Δ12, 14-Prostaglandin J2 Inhibits Osteolytic Breast Cancer Bone Metastasis and Estrogen Deficiency-Induced Bone Loss [PubMed: 25859665] |
| Endocrinology | A role for the calcitonin receptor to limit bone loss during lactation in female mice by inhibiting osteocytic osteolysis [PubMed: 26135836] |
| Tumour Biol | Osteopontin expression in co-cultures of human squamous cell carcinoma-derived cells and osteoblastic cells and its effects on the neoplastic cell phenotype and osteoclastic activation. [Pubmed:27312487] |
| 35 | Hedgehog signaling in bone regulates whole-body energy metabolism through a bone–adipose endocrine relay mediated by PTHrP and adiponectin [pubmed:27740628] |
| Bone | Lactation induces increases in the RANK/RANKL/OPG system in maxillary bone [Pubmed:29382610] |
| International Journal of Molecular Sciences | A Diet Rich in Fish Oil and Leucine Ameliorates Hypercalcemia in Tumour-Induced Cachectic Mice [Pubmed: 31600911] |
| Journal of Orthopaedic Surgery and Research | Identification of a potential gene target for osteoarthritis based on bioinformatics analyses [Pubmed: 32571421] |
| International Immunopharmacology | Parathyroid hormone related protein (PTHrP)-mediated hypercalcemia in malignancy associated with anti-PD-1 immune checkpoint inhibitor treatment and related?… [] |
| Паратиреоидный гормон-родственный протеин как предиктор развития мочекаменной болезни [] | |
| Cell Death Dis | Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer ?- [33510128] |
| Sci Rep | Effects of whole-body vibration training in a cachectic C26 mouse model [34732809] |
| Acta Physiologica | The acute adaptation of renal phosphate transporters in the murine kidney to oral phosphate intake requires multiple signals [Pubmed:35334154] |
