胃蛋白酶(PP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Pepsin (PP)
- 編號UKSA632Hu11
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標記的競爭物、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍2.47-200ng/mL
- 可適靈敏度0.98ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 3591 ¥ 5985 ¥ 10773 ¥ 20948 ¥ 35910
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對胃蛋白酶(PP)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA632Hu01 | 胃蛋白酶(PP)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA632Hu21 | 胃蛋白酶(PP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA632Hu23 | 胃蛋白酶(PP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA632Hu22 | 胃蛋白酶(PP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA632Hu02 | 胃蛋白酶(PP)多克隆抗體 | WB |
| UPAA632Hu08 | 胃蛋白酶(PP)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA632Hu01 | 胃蛋白酶(PP)多克隆抗體 | WB; IHC |
| UPAA632Hu09 | 胃蛋白酶(PP)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA632Hu71 | 胃蛋白酶(PP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA632Hu22 | 胃蛋白酶(PP)單克隆抗體 | WB; IHC |
| USEA632Hu | 胃蛋白酶(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA632Hu | 胃蛋白酶(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA632Hu | 胃蛋白酶(PP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA632Hu | 胃蛋白酶(PP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA632Hu11 | 胃蛋白酶(PP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Radiology and Oncology | LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMA [Szd:Source] |
| Radiology and Oncology | Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer [Pubmed:Pmc4362607] |
| Clin Otolaryngol | Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative study [PubMed: 25516364] |
| journal of pediatric gastroenterology and nutrition | Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants? [PubMed: 25313850] |
| Journal of Voice | Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia [pubmed:28756936] |
| Sensors (Basel) | Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode [Pubmed:29882917] |
| The Tohoku Journal of Experimental Medicine | Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease [] |
| Respiratory?Research | Pulmonary aspiration in preschool children with cystic fibrosis [Pubmed: 30558606] |
| Tanaffos | Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease Exacerbation [Pubmed: 31423141] |
| Sensors | Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux [Pubmed: 31935973] |
| BIOSCIENCE REPORTS | Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasms [Pubmed: 33103719] |
| OTOLARYNGOLOGY-HEAD AND NECK SURGERY | Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia [Pubmed: 32692278] |
| American Journal of Otolaryngology | Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps [Pubmed: 32889371] |
| The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients [] | |
| Med Sci Monit | The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour??- [33220027] |
| Annals of Esophagus | Pepsin properties, structure, and its accurate measurement: a narrative review [] |
| Laryngoscope | The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis [34338331] |
| Pulmonology | Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia [Pubmed:35715334] |
| Neuromodulation: Technology at the Neural Interface | Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome [Pubmed:35088760] |
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