髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Myeloperoxidase (MPO)
- 編號USEA601Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.62ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1588 ¥ 2268 ¥ 10206 ¥ 19278 ¥ 158760
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測髓過氧化物酶(MPO)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的髓過氧化物酶(MPO)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-99 | 94 |
| EDTA plasma(n=5) | 88-102 | 98 |
| heparin plasma(n=5) | 86-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的髓過氧化物酶(MPO)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中髓過氧化物酶(MPO)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-92% | 97-105% | 91-99% | 81-89% |
| EDTA plasma(n=5) | 81-104% | 95-102% | 82-102% | 96-104% |
| heparin plasma(n=5) | 86-103% | 85-96% | 97-105% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將髓過氧化物酶(MPO)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的髓過氧化物酶(MPO)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的髓過氧化物酶(MPO)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的髓過氧化物酶(MPO)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA601Bo01 | 髓過氧化物酶(MPO)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA601Bo21 | 髓過氧化物酶(MPO)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA601Bo01 | 髓過氧化物酶(MPO)多克隆抗體 | WB |
| UMAA601Bo22 | 髓過氧化物酶(MPO)單克隆抗體 | IHC |
| UMAA601Bo21 | 髓過氧化物酶(MPO)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA601Bo | 髓過氧化物酶(MPO)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA601Bo | 髓過氧化物酶(MPO)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| International Journal of Medical Sciences | Ozone Therapy and Hyperbaric Oxygen Treatment in Lung Injury in Septic Rats [PubMed: 21234269] |
| Respiration | Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151] |
| Inflammation | Preventive Effects of Valnemulin on Lipopolysaccharide-Induced Acute Lung Injury in Mice [SpringerLink: 741437t67767777x] |
| Advanced Materials Research | A New Immunosensor for Serum Myeloperoxidase Based on Self-Assembly of Multi-Walled Carbon Nanotubes/Thionine/Gold Nanoparticles-Chitosan [Scientific:1207] |
| 9 | T cells that target carcinoembryonic antigen eradicate orthotopic pancreatic carcinomas without inducing autoimmune colitis in mice [PubMed: 22750462] |
| European Surgical Research | Rosuvastatin Reloading before Cardiac Surgery with Cardiopulmonary Bypass [PubMed: 23406839] |
| Toxicology and Industrial Health | Protective effects of Ankaferd blood stopper on aspirin-induced oxidative mucosal damage in a rat model of gastric injury. [PubMed: 23114375] |
| Experimental Lung Research | Protective effect of pravastatin on lipopolysaccharide-induced acute lung injury during neutropenia recovery in mice [Pubmed: 23368428] |
| International Immunopharmacology | Amentoflavone inhibits iNOS, COX-2 expression and modulates cytokine profile, NF-κB signal transduction pathways in rats with ulcerative colitis [Pubmed: 24126114] |
| Human & experimental toxicology | Neuroprotective effects of α-ketoglutarate and ethyl pyruvate against motor dysfunction and oxidative changes caused by repeated 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine exposure in mice [Pubmed: 23821591] |
| International Immunopharmacology | Protective effect of marine mangrove Rhizophora apiculata on acetic acid induced experimental colitis by regulating anti-oxidant enzymes, inflammatory mediators and nuclear factor-kappa B subunits [ScienceDirect: S1567576913004153] |
| PLOS ONE | Novel CoQ10 Antidiabetic Mechanisms Underlie Its Positive Effect: Modulation of Insulin and Adiponectine Receptors, Tyrosine Kinase, PI3K, Glucose Transporters, sRAGE and Visfatin in Insulin Resistant/Diabetic Rats [Pubmed:24586567] |
| Acta Anaesthesiol Scand. | Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema? [Pubmed:24611481] |
| Journal of Environmental Pathology, Toxicology and Oncology | Protective Effect of?Acacia ferruginea?against Ulcerative Colitis via Modulating Inflammatory Mediators, Cytokine Profile and NF-κB Signal Transduction Pathways [Pubmed:24941292] |
| European Journal of Pharmacology | Neuroprotective effects of rosuvastatin against traumatic spinal cord injury in rats [Pubmed:25084223] |
| Iran J Pharm Res. | Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical Trial [Pubmed:25237354] |
| Chem Biol Interact.? | Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: Contribution of antioxidant, anti-inflammatory and anti-apoptotic activities [Pubmed:25289771] |
| Journal of Surgical Research | Melatonin attenuates intestinal ischemia–reperfusion-induced lung injury in rats by upregulating N-myc downstream-regulated gene 2 [Pubmed:25491174] |
| Neurochemistry International | Sulfiredoxin-1 protects primary cultured astrocytes from ischemia-induced damage [Pubmed:25620665] |
| Ann N Y Acad Sci.? | Modulating effect of Biophytum sensitivum extract on rats with acetic acid-induced ulcerative colitis [Pubmed:5243883] |
| J Crohns | The Syk Inhibitor Fostamatinib Decreases the Severity of Colonic Mucosal Damage in a Rodent Model of Colitis [PubMed: 26116555] |
| Monoclon Antib Immunodiagn Immunother | Preparation of Monoclonal Antibodies and a Simple Myeloperoxidase-Immunosorbent Assay for Detecting Human Myeloperoxidase [Pubmed:26978734] |
| Clinics and Research in Hepatology and Gastroenterology | The efficacy of tyrosine kinase inhibitor dasatinib on colonic mucosal damage in murine model of colitis [Pubmed:26823039] |
| Asian J Pharm Clin Res | THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS [journals:10835] |
| Hepatology. | Toll-like receptor 5 signaling restrains T-cell/natural killer T-cell activation and protects against concanavalin A-induced hepatic injury. [pubmed:28273362] |
| International Journal of Sports Physiology and Performance? | Exploring the Potential of cfDNA Measurements After an Exhaustive Cycle Ergometer Test as a Marker for Performance Related Parameters [pubmed:27617485] |
| Journal of Pharmacy and Pharmaceutical Sciences | Ellagic Acid Protects Against LPS-Induced Acute Lung Injury Through Inhibition of Nuclear Factor Kappa B, Proinflammatory Cytokines and Enhancement of Interleukin-10 [c30adb97b06eb4f27063208df7acb7311368.pdf] |
| Future?Microbiology | Transplantation?of a?bacterial?consortium?ameliorates?trinitrobenzenesulfonic?acid-induced?colitisand?intestinal?dysbiosis?in?rats. [pubmed:27415182] |
| The?Annals?of?Thoracic?Surgery | Sevoflurane?Attenuates?Ischemia-Reperfusion?Injury?in a?Rat?Lung?Transplantation?Model. [pubmed:28190546] |
| Biological Rhythm Research | Fluctuation in the number, type and activity of blood neutrophils in cows exhibiting successful and unsuccessful completion of gestation cycle [09291016.2017.1311984] |
| Journal of Cancer Research and Treatment | Effect of Indole-3-carbinol and/or Metformin on Female Patients with Ulcerative Colitis (Premalignant Condition): Role of Oxidative Stress, Apoptosis and Proinflammatory Cytokines [jcrt:5] |
| Identification of Tengfu Jiangya Tablet Target Biomarkers [journals:ecam] | |
| Hepatology. | Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis. [pubmed:27775820] |
| LAB?ON?A?CHIP | Enzyme?catalysis-electrophoresis?titration?for?multiplex?enzymatic?assay?via?moving?reactionboundary?chip. [pubmed:27464600] |
| Indian Journal of Animal Research | Effect of Poly-herbal mixture and butyric acid feeding on immune parameters of postpartum Murrah buffaloes [publication:299575009] |
| The American Journal of Cardiology | Frequency of Cholesterol Crystals in Culprit Coronary Artery Aspirate During Acute Myocardial Infarction and Their Relation to Inflammation and Myocardial Injury [pubmed:28867129] |
| Evidence-Based Complementary and Alternative Medicine | Identification of Tengfu Jiangya Tablet Target Biomarkers with Quantitative Proteomic Technique [pubmed:28408942] |
| Lasers in Medical Science | Effect of photobiomodulation therapy on reducingthe chemo-induced oral mucositis severity and on salivary levels of CXCL8/interleuki8, nitrite, and myeloperoxidase in patients undergoing hematopoietic stem cell transplantation: a randomized clinical trial [article:10.1007] |
| Experiental And Therapeutic Medicine | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription [pubmed:28672924] |
| Frontiers in Immunology | Upregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells [10.3389:fimmu.2017.00824] |
| Journal of Pediatric Surgery | Does penile tourniquet application alter bacterial adhesion to rat urethral cells: an in vitro study [pubmed:28693848] |
| Food and Agricultural Immunology | Ellagic acid protects against LPS-induced acute lung injury through inhibition of nuclear factor kappa B, proinflammatory cytokines and enhancement of interleukin-10 [10.1080/09540105.2017.1339670] |
| Oxidative Medicine and Cellular Longevity | Expression and Role of the Calcium-Sensing Receptor in Rat Peripheral Blood Polymorphonuclear Neutrophils [pubmed:29081886] |
| Advanced Science Letters | Serum Myeloperoxidase in Stable Coronary Artery Disease [10.1166/asl.2017.8512] |
| Tropical Journal of Pharmaceutical Research | Hepatoprotective effect of taurine and coenzyme Q10 and their combination against acrylamide-induced oxidative stress in rats [10.4314/tjpr.v16i8.14] |
| deposit once | Role of NOD2 and S100A8/S100A9 in the pathogenesis of Coxsackievirus B3-induced myocarditis [10.14279/depositonce-5934] |
| Archives of Medical Research | Effects of Sulphasalazine in Cerebral Ischemia Reperfusion Injury in Rat. [pubmed:28923326] |
| Frontiers in Immunology | The Plasma Kallikrein–Kininogen Pathway is critical in the Pathogenesis of colitis in Mice [Pubmed:29467753] |
| Frontiers in Pharmacology | Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal?… [Pubmed:29375374] |
| Biomedicine & Pharmacotherapy | Evaluation of the anti-hypertensive effect of Tengfu Jiangya tablet by combination of UPLC-Q-exactive-MS-based metabolomics and iTRAQ-based proteomics?… [Pubmed:29453042] |
| Molecular Vision | Resolvin D1 promotes corneal epithelial wound healing and restoration of mechanical sensation in diabetic mice [Pubmed:29643724] |
| Theriogenology | Effect of tropical thermal stress on peri-implantation immune responses in cows [Pubmed:29625402] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Oncology?Letters | Hydrogen?rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus?activated kinase 2/signal transducer and activator of?… [Pubmed:29928398] |
| Journal of Veterinary?Science | Associations of periparturient plasma biochemical parameters, endometrial leukocyte esterase and myeloperoxidase, and bacterial detection with clinical and?… [Pubmed:29332864] |
| Life?Sciences | Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner [Pubmed: 30195035] |
| International?Journal?of?Clinical?and?Experimental?Medicine | Effect of β-glucan on acetic acid-induced colitis in rats [] |
| BioMed Research International | Chlorogenic Acid Attenuates Dextran Sodium Sulfate-Induced Ulcerative Colitis in Mice through MAPK/ERK/JNK Pathway [] |
| Beneficial Microbes | Bacillus licheniformis Zhengchangsheng? attenuates DSS-induced colitis and modulates the gut microbiota in mice [] |
| General Medicine: Open Access | Do Late Phase Hyperbaric and Normobaric Oxygen Therapies have Effect on Liver Damage?: An Experimental Sepsis Model [] |
| journal of surgical research | Posttreatment With LYRM03 Protects Rats From Acute Lung Inflammation Induced by Lipopolysaccharide via Suppressing the NF-κB/MyD88/TLR4 Axis [Pubmed: 31255931] |
| Toxicol Ind Health | Efficacy assessment of co-treated alpha-ketoglutarate and N-acetyl cysteine against the subchronic toxicity of cyanide in rats [Pubmed: 31244408] |
| Journal of Pediatric Surgery | The effects of hydrogen-rich saline solution on intestinal anastomosis performed after intestinal ischemia reperfusion injury [] |
| Biochemical and Biophysical Research Communications | Annexin A1 mimetic peptide Ac2-26 attenuates mechanical injury induced corneal scarring and inflammation [Pubmed: 31519322] |
| Cell Proliferation | SHP2 deficiency promotes Staphylococcus aureus pneumonia following influenza infection [Pubmed: 31782850] |
| Advances in Clinical and Experimental Medicine | Effects of ozone preconditioning on recovery of rat colon anastomosis after preoperative radiotherapy [Pubmed: 31895975] |
| Brain Research | Effects of a high concentration of hydrogen on neurological function after traumatic brain injury in diabetic rats [Pubmed: 31926128] |
| ARTHRITIS RESEARCH & THERAPY | Native myeloperoxidase is required to make the experimental vasculitis model [Pubmed: 31864385] |
| ANALYTICAL CHEMISTRY | Rational Modulation of the Luminescence of Upconversion Nanomaterials with Phycocyanin for the Sensing and Imaging of Myeloperoxidase during an Inflammatory … [Pubmed: 32126753] |
| RESEARCH IN VETERINARY SCIENCE | Peripartum changes in the activity and expression of neutrophils may predispose to the postpartum occurrence of metritis in dairy cows [Pubmed: 33229058] |
| RESPIRATORY RESEARCH | The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation?… [Pubmed: 33148285] |
| J Toxicol Sci | Lead exposure induces cell autophagy via blocking the Akt/mTOR signaling in rat astrocytes [Pubmed: 32879255] |
| Theriogenology | A comparative study on various immunological parameters influencing embryo survivability in crossbred dairy cows [Pubmed: 32810791] |
| Scientific Reports | Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cows [Pubmed: 32636462] |
| INTERNATIONAL IMMUNOPHARMACOLOGY | Gambogic acid alleviates inflammation and apoptosis and protects the blood-milk barrier in mastitis induced by LPS [Pubmed: 32585608] |
| Cells | Protective Effects of SIRT6 Overexpression against DSS-Induced Colitis in Mice [Pubmed: 32580272] |
| Legal Medicine | DNA microarray analysis of hypothermia-exposed murine lungs for identification of forensic biomarkers [Pubmed: 33161360] |
| INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | Juglanin administration protects skin against UVB?induced injury by reducing Nrf2?dependent ROS generation [Pubmed: 32377697] |
| respiratory research | The adverse alterations of HDL in quality promote septic ARDS via exacerbated pulmonary endothelial dysfunction [] |
| JOURNAL OF SURGICAL RESEARCH | Ameliorating Effects of |?-glucan on Epigastric Artery Island Flap Ischemia-Reperfusion Injury [33477077] |
| Stem Cell Research & Therapy | Dental pulp stem cells overexpressing hepatocyte growth factor facilitate the repair of DSS-induced ulcerative colitis [33413675] |
| Neural Regeneration Research | Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury [33433486] |
| J Physiol Biochem | Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model [33595775] |
| J Pediatr Urol | Protective effects of hydrogen rich saline solution on ventral penile Mathieu type flap with penile tourniquet application in rats [33608226] |
| J Reprod Immunol | JAK3 and PI3K mediates the suppressive effects of interferon tau on neutrophil extracellular traps formation during peri-implantation period [33862433] |
| Br J Pharmacol | Macrophage Extracellular Traps Aggravate Iron Overload?\Related Liver Ischemia/Reperfusion Injury [33959955] |
| Nephron | Characterization of a Rat Model of Myeloperoxidase-Anti-Neutrophil Cytoplasmic Antibody-Associated Crescentic Glomerulonephritis [33910203] |
| Exp Eye Res | Topical calcitriol application promotes diabetic corneal wound healing and reinnervation through inhibiting NLRP3 inflammasome activation [34144035] |
| Sci Rep | Daphnetin ameliorates acute lung injury in mice with severe acute pancreatitis by inhibiting the JAK2¨CSTAT3 pathway [34075160] |
| researchsquare | |ê?opioid receptor agonist U50488H attenuates postoperative cognitive dysfunction of cardiopulmonary bypass rats through the PI3K/AKT/Nrf2/HO?1 pathway [33649775] |
| J Inflamm Res | Novel 1, 3, 4-Oxadiazole Derivatives of Pyrrolo [3, 4-d] Pyridazinone Exert Anti-Inflammatory Activity without Acute Gastrotoxicity in the Carrageenan-Induced?… [34754217] |
| World J Gastroenterol | Calycosin attenuates severe acute pancreatitis-associated acute lung injury by curtailing high mobility group box 1-induced inflammation [34908806] |
| Experimental and Therapeutic Medicine | Blocking tumor necrosis factor-α delays progression of chronic obstructive pulmonary disease in rats through inhibiting MAPK signaling pathway and activating?… [34630665] |
| Preprints | Hyperactive neutrophils infiltrate vital organs of tumor bearing host and contribute to gradual systemic deterioration with tumor progression [34890699] |
| J Dairy Sci | Saccharomyces cerevisiae fermentation products reduce bacterial endotoxin concentrations and inflammation during grain-based subacute ruminal acidosis?… [Pubmed:34998547] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
| Reports of Biochemistry & Molecular Biology | Serum Myeloperoxidase as A Biomarker of Asthma Severity Among Adults: A Case Control Study [Pubmed:35765520] |
| Journal of Functional Foods | Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways [] |
| A Novel Role of Tinospora Cordifolia in Amelioration of Cancer-Induced Systemic Deterioration By Taming Neutrophil Infiltration and Hyperactivation [] |
留言咨詢
