胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
- 編號USEA853Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.625-40ng/mL
- 靈敏度最小可檢測劑量小于等于0.244ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1176 ¥ 1680 ¥ 7560 ¥ 14280 ¥ 117600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測胱天蛋白酶8(CASP8)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將胱天蛋白酶8(CASP8)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的胱天蛋白酶8(CASP8)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的胱天蛋白酶8(CASP8)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的胱天蛋白酶8(CASP8)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA853Hu02 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA853Hu03 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA853Hu01 | 胱天蛋白酶8(CASP8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA853Hu02 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; ICC/IF |
| UPAA853Hu04 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB |
| UPAA853Hu01 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA853Hu03 | 胱天蛋白酶8(CASP8)多克隆抗體 | WB; IHC; ICC/IF |
| UFAA853Hu01 | 抗胱天蛋白酶8(CASP8)多克隆抗體 | FCM |
| ULAA853Hu81 | 胱天蛋白酶8(CASP8)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA853Hu71 | 胱天蛋白酶8(CASP8)多克隆抗體(生物素標記) | WB |
| UMAA853Hu21 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC |
| UMAA853Hu23 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB; IHC |
| UMAA853Hu25 | 胱天蛋白酶8(CASP8)單克隆抗體 | IHC |
| UMAA853Hu24 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| UMAA853Hu29 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| UMAA853Hu27 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| UMAA853Hu22 | 胱天蛋白酶8(CASP8)單克隆抗體 | WB |
| USEA853Hu | 胱天蛋白酶8(CASP8)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA853Hu | 胱天蛋白酶8(CASP8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA853Hu01 | 胱天蛋白酶8(CASP8)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
| Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells [Pubmed:29555410] |
| Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336] |
| Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats [Pubmed: 31421311] |
| Journal of Biochemical and Molecular Toxicology | Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation [Pubmed: 32045101] |
| life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats [Pubmed: 32259601] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
| Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling [Pubmed: 33199237] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways [Pubmed: 32942000] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low? dose of?… [Pubmed: 32915453] |
| J Biochem Mol Toxicol | Tranilast abrogates cisplatin?\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation [34047436] |
