粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- 編號USEA756Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍78-5,000pg/mL
- 靈敏度最小可檢測劑量小于等于30pg/mL.
- 樣本類型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1323 ¥ 1890 ¥ 8505 ¥ 16065 ¥ 132300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測粘蛋白5AC(MUC5AC)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將粘蛋白5AC(MUC5AC)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的粘蛋白5AC(MUC5AC)與連接于固相載體上的抗體結合，然后加入生物素化的粘蛋白5AC(MUC5AC)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的粘蛋白5AC(MUC5AC)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA756Hu01 | 粘蛋白5AC(MUC5AC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA756Hu01 | 粘蛋白5AC(MUC5AC)多克隆抗體 | IHC |
| ULAA756Hu81 | 粘蛋白5AC(MUC5AC)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA756Hu71 | 粘蛋白5AC(MUC5AC)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAA756Hu21 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB |
| UMAA756Hu22 | 粘蛋白5AC(MUC5AC)單克隆抗體 | WB |
| ULAA756Hu72 | 粘蛋白5AC(MUC5AC)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA756Hu | 粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA756Hu | 粘蛋白5AC(MUC5AC)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA756Hu | 粘蛋白5AC(MUC5AC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA756Hu01 | 粘蛋白5AC(MUC5AC)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA756Hu01 | 粘蛋白5AC(MUC5AC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Annals of surgical oncology | Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. [PubMed: 20717734] |
| Investigative Ophthalmology & Visual Science | Entrapment of Conjunctival Goblet Cells by Desiccation-Induced Cornification [PubMed: 21421863] |
| Cell & Molecular Biology | Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in mice [PubMed: 20550619] |
| Mucosal Immunology | Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13 [Nature: 201082a] |
| European Journal of Pharmacology | Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways [ScienceDirect: S0014299912005523] |
| PLoS One | Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway Inflammation [PubMed: PMC3639905] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780] |
| Surgery | A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) [Pubmed: 24468034] |
| Biochemical and Biophysical Research Communications | The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease [Pubmed:24291709] |
| Journal of Proteomics | Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction [Pubmed:24726482] |
| Experimental Lung Research | The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway [Pubmed:24901072] |
| Surgery.? | Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance [Pubmed:25151557] |
| Allergology International | Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells [Pubmed:25838093] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851] |
| S | Isabela Casagrande Jeremias [Teses: Disponiveis] |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164] |
| Deletion Polymorphism and Chronic Obstructive Pulmonary Disease | ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells [Pubmed:27075970] |
| Int J Clin Exp Med | The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai [files:ijcem0020163.pdf] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513] |
| Sci Rep | Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers [Pubmed:27297822] |
| Cytokine. | Interleukin-33?induces?mucin?gene?expression?and?goblet?cell?hyperplasia?in?human?nasalepithelial?cells. [pubmed:27776277] |
| J Virol.? | Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective. [pubmed:28275196] |
| Respiratory Physiology & Neurobiology | Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway. [pubmed:27884794] |
| Journal of Immunology Research | Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse models [pubmed:28835901] |
| BASIC INVESTIGATION | Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseases [pubmed:28644234] |
| International Immunopharmacology | Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathway [pubmed:28550735] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158] | |
| Cellular Microbiology | An adherent mucus layer attenuates the genotoxic effect of colibactin. [pubmed:29156489] |
| Respiratory Research | IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells [pubmed:28931396] |
| Chemotherapy | Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells. [pubmed:28578323] |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623] |
| European?Journal of Pharmacology? | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534] |
| Inflammation | Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in?… [Pubmed:29349683] |
| Pulmonary Pharmacology and Therapeutics | In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extracts [Pubmed:29698781] |
| European Respiratory Society | Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion [Pubmed:29946002] |
| Clinical?and?Experimental?Ophthalmology | Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips [Pubmed: 30214141] |
| international journal of biological macromolecules | Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer [Pubmed: 30287374] |
| Journal of Investigative?Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494] |
| Phytomedicine | Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion [Pubmed: 31301536] |
| Scientific Reports | Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5AC [Pubmed: 32076085] |
| Eur J Pharmacol | TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK?… [Pubmed: 33068589] |
| journal of ethnopharmacology | Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K … [Pubmed: 32344236] |
| Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice [] | |
| Exp Mol Pathol | The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal??- [33285209] |
| FEBS Open Bio | Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells [33332733] |
| Cell Rep | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs [33535050] |
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