腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Fatty Acid Binding Protein 2, Intestinal (FABP2)
FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein
- 編號USEA559Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.133ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1277 ¥ 1824 ¥ 8208 ¥ 15504 ¥ 127680
- 欲了解實際交易價格和更多情況，請與當?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標準曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測腸型脂肪酸結(jié)合蛋白(FABP2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的腸型脂肪酸結(jié)合蛋白(FABP2)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 88-102 | 92 |
| EDTA plasma(n=5) | 98-105 | 101 |
| heparin plasma(n=5) | 80-96 | 92 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的腸型脂肪酸結(jié)合蛋白(FABP2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中腸型脂肪酸結(jié)合蛋白(FABP2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-93% | 79-101% | 79-98% | 82-96% |
| EDTA plasma(n=5) | 79-98% | 97-104% | 94-101% | 78-92% |
| heparin plasma(n=5) | 80-101% | 81-97% | 78-103% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將腸型脂肪酸結(jié)合蛋白(FABP2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的腸型脂肪酸結(jié)合蛋白(FABP2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的腸型脂肪酸結(jié)合蛋白(FABP2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的腸型脂肪酸結(jié)合蛋白(FABP2)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA559Ra01 | 腸型脂肪酸結(jié)合蛋白(FABP2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA559Ra01 | 腸型脂肪酸結(jié)合蛋白(FABP2)多克隆抗體 | WB; IHC |
| ULAA559Ra71 | 腸型脂肪酸結(jié)合蛋白(FABP2)多克隆抗體(生物素標記) | WB |
| ULAA559Ra81 | 腸型脂肪酸結(jié)合蛋白(FABP2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA559Ra22 | 腸型脂肪酸結(jié)合蛋白(FABP2)單克隆抗體 | WB |
| UMAA559Ra24 | 腸型脂肪酸結(jié)合蛋白(FABP2)單克隆抗體 | WB; IHC |
| UMAA559Ra23 | 腸型脂肪酸結(jié)合蛋白(FABP2)單克隆抗體 | WB; IHC |
| UMAA559Ra21 | 腸型脂肪酸結(jié)合蛋白(FABP2)單克隆抗體 | WB; IHC |
| USEA559Ra | 腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA559Ra | 腸型脂肪酸結(jié)合蛋白(FABP2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA559Ra01 | 腸型脂肪酸結(jié)合蛋白(FABP2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Food Chem | Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883] |
| Oxid Med Cell Longev | Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation Model [PubMed: 26064429] |
| Vet Immunol Immunopathol | Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis. [pubmed:28895864] |
| PLoS One | Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed rats [pubmed:28622357] |
| Journal of Immunology and Immunopathology | Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function [10.5958/0973-9149.2017.00011.9] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286] |
| European?Journal of Clinical Nutrition | Lack of liver steatosis in germ-free mice following hypercaloric diets [Pubmed:29926176] |
| Nutrients | Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and Iron [Pubmed:29861488] |
| International?Surgery?Journal | Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemia [Doi: 10.18203/2349-2902.isj20190058] |
| Hepatology?Communications | Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial [] |
| Tropical?Animal?Health?and?Production | Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized?… [Pubmed: 30790158] |
| Biological & Pharmaceutical Bulletin | Role of Aquaporin-3 in Intestinal Injury Induced by Sepsis [Pubmed: 31582652] |
| International Journal of Medical Sciences | FABP1 and FABP2 as markers of diabetic nephropathy [Pubmed: 32922200] |
| Int Immunopharmacol | Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory response [Pubmed: 32361189] |
| Journal of Exercise Physiology Online | Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period. [] |
| The Journal of Nutritional Biochemistry | Effects of fucoidan on tumor prevention and gut flora [] |
| european journal of clinical nutrition? | Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury [33514869] |
| J Cell Mol Med | The |á2AR/Caveolin?\1/p38MAPK/NF?\|êB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia?\reperfusion [34114328] |
| BMC Gastroenterol | Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity [34022802] |
| JOURNAL OF APPLIED PHYSIOLOGY | Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice [34528459] |
| PLoS One | The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsis [Pubmed:35576220] |
