Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)
Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)
P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide
- 編號(hào)UIEA957Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)1h, 10min
- 檢測(cè)范圍-
- 靈敏度-
- 樣本類型-
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2205 ¥ 3150 ¥ 14175 ¥ 26775 ¥ 220500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-103 | 101 |
| EDTA plasma(n=5) | 78-97 | 90 |
| heparin plasma(n=5) | 83-94 | 87 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-103% | 89-101% | 82-93% | 95-105% |
| EDTA plasma(n=5) | 90-97% | 95-104% | 81-98% | 84-91% |
| heparin plasma(n=5) | 80-92% | 78-102% | 87-99% | 94-103% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測(cè)溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測(cè)溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標(biāo)準(zhǔn)品或標(biāo)本以及同時(shí)加入HRP標(biāo)記Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)的多克隆抗體，孵育結(jié)束徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的被檢測(cè)物濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA957Hu61 | Ⅰ型前膠原氨基端原肽(PINP)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA957Hu61 | Ⅰ型前膠原氨基端原肽(PINP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USPA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
| UCPA957Hu12 | Ⅰ型前膠原氨基端原肽(PINP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA957Hu32 | Ⅰ型前膠原氨基端原肽(PINP)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA957Hu31 | Ⅰ型前膠原氨基端原肽(PINP)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA957Hu21 | Ⅰ型前膠原氨基端原肽(PINP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| USPA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)合成多肽 | Immunogen; Blocking Peptide. |
| UCPA957Hu11 | Ⅰ型前膠原氨基端原肽(PINP)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA957Hu08 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | IHC |
| UPAA957Hu09 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB |
| UPAA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB |
| UPAA957Hu02 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA957Hu81 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA957Hu71 | Ⅰ型前膠原氨基端原肽(PINP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB |
| URAA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| URAA957Hu22 | Ⅰ型前膠原氨基端原肽(PINP)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA957Hu24 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB |
| UMAA957Hu21 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB |
| UMAA957Hu28 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB |
| UMAA957Hu23 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | IHC |
| UMAA957Hu26 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | ICC/IF |
| UMAA957Hu25 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA957Hu27 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA957Hu29 | Ⅰ型前膠原氨基端原肽(PINP)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA957Hu | Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA957Hu | Ⅰ型前膠原氨基端原肽(PINP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA957Hu11 | Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UKSA957Hu01 | Ⅰ型前膠原氨基端原肽(PINP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
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| Research square | 4-Hexylresorcinol Inhibits Osteoclastogenesis by Suppressing NF‐κB Signaling Pathway and Reverses Bone Loss in Ovariectomized Mice [] |
| 淫羊藿水提物及提取藥渣的抗骨質(zhì)疏松藥效比較研究 [] | |
| Pamukkale T?p Dergisi | Postmenapozal Tip-2 diyabetes mellitus' lu hastalarda osteoporoza ba?l? k?r?k riskinin ve kemik Turnoveri'nin de?erlendirilmesi [] |
| International Journal of Pharmaceutical and Clinical Research | Bone Turnover Markers in Osteogenesis Imperfecta and Effect of Bisphosphonate Treatment: First Egyptian Study [] |
| Science Advances | A biomimetically hierarchical polyetherketoneketone scaffold for osteoporotic bone repair [33310848] |
| Rheumatology | Proinflammatory and bone protective role of calcitonin gene-related peptide alpha in collagen antibody-induced arthritis [33221885] |
| Nutrients | Low Energy Availability with and without a High-Protein Diet Suppresses Bone Formation and Increases Bone Resorption in Men: A Randomized Controlled Pilot ?- [33671093] |
| J Bone Miner Res | Lgr4 promotes aerobic glycolysis and differentiation in osteoblasts via the canonical Wnt/|??\catenin pathway [33950533] |
| Phytomedicine | Randomized, double-blind, placebo-controlled trial to examine the safety, pharmacokinetics and effects of Epimedium prenylflavonoids, on bone specific alkaline?… [34352588] |
| Nutrients | The Effect of Daily Methylsulfonylmethane (MSM) Consumption on High-Density Lipoprotein Cholesterol in Healthy Overweight and Obese Adults: A Randomized?… [34684621] |
| PLoS One | Osteoblast-specific inactivation of p53 results in locally increased bone formation [34793446] |
| Bone Res | The WNT1G177C mutation specifically affects skeletal integrity in a mouse model of osteogenesis imperfecta type XV [34759273] |
| Nutrients | The Effect of Caloric Restriction with and without n-3 PUFA Supplementation on Bone Turnover Markers in Blood of Subjects with Abdominal Obesity: A Randomized?… [34578973] |
| Chronobiol Int | Night shift work and osteoporosis-bone turnover markers among female blue-collar workers in Poland [Pubmed:35139709] |
| Cancers (Basel) | Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer Cells [Pubmed:35158823] |
| Cell Death Discov | BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis [Pubmed:35523787] |
| Experimental and Molecular Medicine | Foxf2 represses bone formation via Wnt2b/β-catenin signaling [Pubmed:35668101] |
| Bioactive Materials | Osteoporotic bone recovery by a bamboo-structured bioceramic with controlled release of hydroxyapatite nanoparticles [Pubmed:35386445] |
| Journal of Orthopaedic Translation | Puerarin specifically disrupts osteoclast activation via blocking integrin-β3 Pyk2/Src/Cbl signaling pathway [Pubmed:35228997] |
| Bioactive Materials | Construction of a magnesium hydroxide/graphene oxide/hydroxyapatite composite coating on Mg–Ca–Zn–Ag alloy to inhibit bacterial infection and promote?… [Pubmed:35585377] |
| Journal of Orthopaedic Translation | Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35?… [] |
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