血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Angiotensin I Converting Enzyme (ACE) ,etc. by FLIA (Flow Luminescence Immunoassay)
CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
（注：單次混測多因子不超過8個指標 ）
- 編號ULMA004Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.02-25ng/mL
- 靈敏度最小可檢測劑量小于等于0.007 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-90 | 85 |
| EDTA plasma(n=5) | 94-105 | 98 |
| heparin plasma(n=5) | 89-96 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-97% | 87-101% | 79-96% | 82-97% |
| EDTA plasma(n=5) | 80-97% | 99-105% | 82-92% | 78-92% |
| heparin plasma(n=5) | 87-99% | 79-103% | 88-104% | 87-97% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠）標準品或樣本100μL及磁珠10μL，
37°C酶標板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA004Hu01 | 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA004Hu01 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 | WB; IHC |
| UPAA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 | WB |
| ULAA004Hu81 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| ULAA004Hu71 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體(生物素標記) | IHC |
| UMAA004Hu22 | 血管緊張素轉(zhuǎn)化酶(ACE)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA004Hu21 | 血管緊張素轉(zhuǎn)化酶(ACE)單克隆抗體 | WB |
| USEA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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| Journal of Cancer Therapy | Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source] |
| The American journal of clinical nutrition | Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151] |
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| biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636] |
| Biomedicine & Pharmacotherapy | Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471] |
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| Journal of?Surgical?Research | Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841] |
| Intensive Care Medicine | Angiotensin-converting enzymes in acute respiratory distress syndrome [] |
| International Journal of Biological Sciences | Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway [] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181] |
| American Journal of Respiratory and Critical Care Medicine | Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511] |
| Int Immunopharmacol | Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215] |
| Food Bioscience | Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension [] |
| Journal of Extracellular Vesicles | MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme?… [] |
| Biotechnology and Pharmacy | Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats [] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… [] |
