18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Cathelicidin Antimicrobial Peptide (CAMP) ,etc. by FLIA (Flow Luminescence Immunoassay)
CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號ULMC419Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.05-50ng/mL
- 靈敏度最小可檢測劑量小于等于0.017 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 91-98 | 94 |
| heparin plasma(n=5) | 87-94 | 91 |
| sodium citrate plasma(n=5) | 96-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-91% | 87-99% | 86-93% | 82-101% |
| EDTA plasma(n=5) | 90-99% | 85-92% | 80-92% | 90-99% |
| heparin plasma(n=5) | 82-101% | 80-92% | 79-89% | 80-88% |
| sodium citrate plasma(n=5) | 84-94% | 84-102% | 83-92% | 82-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPC419Mu61 | 18kDa陽離子抗菌肽(CAMP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC419Mu02 | 18kDa陽離子抗菌肽(CAMP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC419Mu01 | 18kDa陽離子抗菌肽(CAMP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC419Mu61 | 18kDa陽離子抗菌肽(CAMP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAC419Mu02 | 18kDa陽離子抗菌肽(CAMP)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAC419Mu01 | 18kDa陽離子抗菌肽(CAMP)多克隆抗體 | WB; IHC |
| USEC419Mu | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEC419Mu | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMC419Mu | 18kDa陽離子抗菌肽(CAMP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSC419Mu01 | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UKSC419Mu11 | 18kDa陽離子抗菌肽(CAMP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Urology | Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells [PubMed: 25681250] |
| Sarcoidosis Vasc Diffuse Lung Dis | Cathelicidin as a link between sarcoidosis and tuberculosis [PubMed: 26422567] |
| Allergy and Asthma Proceedings | The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children [PubMed: 26108071] |
| J Clin Microbiol. | Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis. [Pubmed:27008883] |
| experimental dermatology | Light-emitting diodes downregulate cathelicidin, kallikrein and toll-like receptor 2 expressions in keratinocytes and rosacea-like mouse skin. [pubmed:27315464] |
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| Experimental Parasitology | Antimalarial activity of vitamin D3 (VD3) does not result from VD3-induced antimicrobial agents including nitric oxide or cathelicidin [Pubmed: 30904694] |
| Theranostics | Myeloid cell-derived LL-37 promotes lung cancer growth by activating Wnt/β-catenin signaling [Pubmed: 31149039] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37 [Pubmed: 32050475] |
| MICROB PATHOGENESIS | Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy [Pubmed: 32645421] |
| International Journal of Nanomedicine | Effect of Lonicerae japonicae Flos Carbonisata-Derived Carbon Dots on Rat Models of Fever and Hypothermia Induced by Lipopolysaccharide [Pubmed: 32606669] |
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| Type I interferons link skin-associated dysbiotic commensal bacteria to pathogenic inflammation and angiogenesis in rosacea [] | |
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