熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Heat Shock Protein 70 (HSP70) ,etc. by FLIA (Flow Luminescence Immunoassay)
DnaK; 70 Kilodalton Heat Shock Proteins
（注：?jiǎn)未位鞙y(cè)多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)ULMA873Mi
- 物種Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍0.2-200ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.067 ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒對(duì)檢測(cè)熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)具有較高的靈敏度。
經(jīng)檢測(cè)牛、山羊、綿羊之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-96 | 89 |
| EDTA plasma(n=5) | 78-89 | 84 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-89% | 78-103% | 90-98% | 84-96% |
| EDTA plasma(n=5) | 82-96% | 87-94% | 92-101% | 82-89% |
| heparin plasma(n=5) | 80-99% | 85-93% | 94-101% | 98-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| USEA873Mi | 熱休克蛋白70(HSP70)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA873Mi | 熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Molecular Medicine | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy [SpringerLink: b8738j3571287425] |
| Journal of Neuroinflammation | Neuroinflammation in autism spectrum disorders [PubMed: PMC3549857] |
| Eur Arch Psychiatry Clin Neurosci. | Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder [Pubmed: 23455589] |
| Arch Bronconeumol | Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue [PubMed: 26055465] |
| Arch Bronconeumol | Heat Shock Proteins, [PubMed: 26055465] |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
| Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955] |
| Plos One | Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model [Pubmed:27144532] |
| J Pak Med Assoc. | Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine [mnstemps:33-1429777310] |
| Egyptian Poultry Science Journal | STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN [abstract:3d115259031] |
| Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load [S0144860916301935] | |
| Animal?Science?Journal | Prokaryotic?expression?of?chicken?interferon-γ?fusion?protein?and its?effect?on?expression?ofpoultry?heat?shock?protein?70?under?heat?stress. [pubmed:27753175] |
| Pharmacol Rep | High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male?rat?liver. [pubmed:28531762] |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311] |
| Immunobiology | Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer [pubmed:29111316] |
| Egyptian Poultry Science Journal | EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. [125788660] |
| Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death [DOI:10.1016/j.biopha.2017.01.087] |
| Egyptian Poultry Science Journal | IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. [ISSN: 1110-5623] |
| Aquaculture | Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems [10.1016/j.aquaculture.2017.11.030] |
| Journal of dairy research | Detection of 70 kDa heat shock protein in the saliva of dairy cows. [pubmed:28625184] |
| Journal of animal science | Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers [pubmed:29293755] |
| Biochemistry and?Cell?Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy [Pubmed:29365285] |
| Egyptian Poultry Science Journal | THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT?… [article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf] |
| Egyptian Poultry Science Journal | EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS?… [:] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker [] |
| Biological Procedures Online | Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells [] |
| Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer [] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains [Pubmed: 31468353] |
| ACTA TROPICA | Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis [Pubmed: 33221280] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Slovak Journal of Animal Science | EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA [] |
| 博士論文 | Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and?… [] |
| Translational Medicine Communications | Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory … [] |
| Physiological impacts of suspended solids in recirculating aquaculture [] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces [34091792] |
| Journal of Thermal Biology | Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress [33627283] |
| J Korean Neurosurg Soc | The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model [34376039] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats [Pubmed:35421180] |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood [] |
