神經元特異性烯醇化酶(NSE)單克隆抗體
Monoclonal Antibody to Enolase, Neuron Specific (NSE)
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
- 編號UMAA537Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C12
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPA537Hu01-神經元特異性烯醇化酶(NSE)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; ICC/IF
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 302 ¥ 706 ¥ 1008 ¥ 2520 ¥ 10080
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產品包裝(模擬)
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產品包裝(模擬)
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IHC圖
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AF488 staining on IF;
Sample: SH-SY5Y cell
Primary Ab: 30μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11) -
FITC staining on IF;
Sample: HepG2 cell
Primary Ab: 20μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Porcine Cerebrum lysate Primary Ab: 0.1μg/ml Mouse Anti-Human NSE Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Fetal Rat Cerebrum lysate
Primary Ab: 0.1μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: A549 cell lysate; Lane3: SH-SY5Y cell lysate; Lane4: U87MG cell lysate; Lane5: PC-12 cell lysate;<br/>Primary Ab: 0.1μg/ml Mouse Anti-Human NSE Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
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Western Blot; Sample: Rat Cerebrum lysate;
Primary Ab: 3μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 3μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 3μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Human Lung lysate;
Primary Ab: 3μg/ml Mouse Anti-Human NSE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對NSE的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別NSE。
用法
Western blotting: 0.5-3μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
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| URPA537Hu01 | 神經元特異性烯醇化酶(NSE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA537Hu01 | 神經元特異性烯醇化酶(NSE)多克隆抗體 | WB; IHC |
| UPAA537Hu02 | 神經元特異性烯醇化酶(NSE)多克隆抗體 | WB |
| ULAA537Hu71 | 神經元特異性烯醇化酶(NSE)多克隆抗體(生物素標記) | WB |
| ULAA537Hu81 | 神經元特異性烯醇化酶(NSE)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA537Hu27 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB |
| UMAA537Hu29 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu25 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu21 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu22 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu23 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA537Hu26 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| UMAA537Hu28 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; ICC/IF |
| UMAA537Hu24 | 神經元特異性烯醇化酶(NSE)單克隆抗體 | WB; IHC |
| USEA537Hu | 神經元特異性烯醇化酶(NSE)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA537Hu | 神經元特異性烯醇化酶(NSE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Journal of the Neurological?Sciences | S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid?… [Pubmed:29801873] |
| Iranian Journal of Veterinary Research | Nitrosative stress indices in dogs with neurological form of canine distemper [Doi: 10.22099/IJVR.2018.4943] |
| Evidence-Based Complementary and Alternative Medicine | Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway [] |
| The?Eurasian?Journal?of?Medicine | Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the?… [] |
| ACTA NEUROBIOLOGIAE EXPERIMENTALIS | Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion?… [Pubmed: 32214270] |
| Stem Cells International | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2?… [Pubmed: 32300366] |
| European Review for Medical and Pharmacological Sciences | Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … [Pubmed: 32432773] |
| Neurobiology of Learning and Memory | Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats [] |
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