熱休克蛋白70(HSP70)單克隆抗體
Monoclonal Antibody to Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
- 編號(hào)UMAA873Hu25
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C5
- Ig亞型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 -
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 308 ¥ 718 ¥ 1026 ¥ 2565 ¥ 10260
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: A431 cell lysate;
Primary Ab: 0.2μg/ml Mouse Anti-Human HSP70 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)HSP70的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別HSP70。
用法
Western blotting: 0.1-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA873Hu61 | 熱休克蛋白70(HSP70)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA873Hu61 | 熱休克蛋白70(HSP70)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA873Hu01 | 熱休克蛋白70(HSP70)多克隆抗體 | WB |
| ULAA873Hu71 | 熱休克蛋白70(HSP70)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA873Hu81 | 熱休克蛋白70(HSP70)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA873Hu21 | 熱休克蛋白70(HSP70)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA873Hu22 | 熱休克蛋白70(HSP70)單克隆抗體 | WB |
| UMAA873Hu23 | 熱休克蛋白70(HSP70)單克隆抗體 | WB |
| UMAA873Hu24 | 熱休克蛋白70(HSP70)單克隆抗體 | WB |
| UMAA873Hu25 | 熱休克蛋白70(HSP70)單克隆抗體 | WB |
| UMEA873Hu | 熱休克蛋白70(HSP70)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA873Hu | 熱休克蛋白70(HSP70)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA873Hu | 熱休克蛋白70(HSP70)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Molecular Medicine | Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy [SpringerLink: b8738j3571287425] |
| Journal of Neuroinflammation | Neuroinflammation in autism spectrum disorders [PubMed: PMC3549857] |
| Eur Arch Psychiatry Clin Neurosci. | Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder [Pubmed: 23455589] |
| Arch Bronconeumol | Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue [PubMed: 26055465] |
| Arch Bronconeumol | Heat Shock Proteins, [PubMed: 26055465] |
| Animal Science Journal | The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells [PubMed: 26467738] |
| Transplant Proc | L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955] |
| Plos One | Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model [Pubmed:27144532] |
| J Pak Med Assoc. | Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine [mnstemps:33-1429777310] |
| Egyptian Poultry Science Journal | STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN [abstract:3d115259031] |
| Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load [S0144860916301935] | |
| Animal?Science?Journal | Prokaryotic?expression?of?chicken?interferon-γ?fusion?protein?and its?effect?on?expression?ofpoultry?heat?shock?protein?70?under?heat?stress. [pubmed:27753175] |
| Pharmacol Rep | High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male?rat?liver. [pubmed:28531762] |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311] |
| Immunobiology | Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer [pubmed:29111316] |
| Egyptian Poultry Science Journal | EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. [125788660] |
| Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death [DOI:10.1016/j.biopha.2017.01.087] |
| Egyptian Poultry Science Journal | IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. [ISSN: 1110-5623] |
| Aquaculture | Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems [10.1016/j.aquaculture.2017.11.030] |
| Journal of dairy research | Detection of 70 kDa heat shock protein in the saliva of dairy cows. [pubmed:28625184] |
| Journal of animal science | Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers [pubmed:29293755] |
| Biochemistry and?Cell?Biology | Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy [Pubmed:29365285] |
| Egyptian Poultry Science Journal | THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT?… [article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf] |
| Egyptian Poultry Science Journal | EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS?… [:] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
| Journal of Biological Sciences | Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker [] |
| Biological Procedures Online | Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells [] |
| Journal of Biological Sciences | Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer [] |
| 博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
| environmental science and pollution research | Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains [Pubmed: 31468353] |
| ACTA TROPICA | Oxidative stress mediated apoptotic potential of mefloquine on experimental trichinellosis [Pubmed: 33221280] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Slovak Journal of Animal Science | EFFECTS OF HEAT STRESS ON PHYSIOLOGICAL PARAMETERS AND SERUM CONCENTRATION OF HSP70 IN INDIGENOUS BREEDS OF SHEEP IN NIGERIA [] |
| 博士論文 | Effect of organic acid blend and prebiotic on growth performance gut histomorphometry, tibia bone characteristics, reactive oxygen species, microbial count and?… [] |
| Translational Medicine Communications | Concurrent high-intensity aerobic and resistance exercise modulates systemic release of alarmins (HMGB1, S100A8/A9, HSP70) and inflammatory … [] |
| Physiological impacts of suspended solids in recirculating aquaculture [] | |
| Clin Oral Investig | Evaluation of heat shock protein 70 and toll-like receptor 4 expression in gingival crevicular fluid in response to orthodontic forces [34091792] |
| Journal of Thermal Biology | Effects of L-proline on cellular responses of hen erythrocytes subjected to thermal stress [33627283] |
| J Korean Neurosurg Soc | The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model [34376039] |
| PLoS One | Neuroprotective effect of sodium alginate against chromium-induced brain damage in rats [Pubmed:35421180] |
| Med Science | Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood [] |
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